what is another indicator found in dnase media

With the doubling of population and changing lifestyle pattern of the inhabitants the quantity of municipal waste generated is increasing in an alarming rate. Waste generation and its control have taken an important role in our environment.

Keywords: waste dump sites, bacteria, antagonism, extracellular enzymes Introduction These results have increased the scope of finding industrially important bacteria from municipal waste dump sites and these isolates could be vital source for the discovery of industrially useful enzymes/molecules. All of the 6 strains capable of producing protease were also applied for waste degradation efficiency test. Among the 9 isolates 6 exhibited protease production and 1 was selected for protease production with high titer value. The optimal cultural conditions, microbiological characteristics, biochemical characteristics, antagonistic and synergistic activities within the strains, tolerance to 5 heavy metals (arsenic, zinc, lead, mercury, and cadmium), their sensitivity towards four different antibiotics (gentamycin, oxytetracycline, penicillin, streptomycin) and production of extracellular enzyme of the bacterial strains were documented. The two indicator media should be useful in investigations of the microbial degradation of other acid-yielding halogenated pesticides.A total of 10 samples were collected from waste dump sites of Dhapa and Barrackpore Municipality and 9 isolates of bacteria were isolated using Nutrient Agar medium. Acid production from the herbicide, 4-chloro-2-methylphenoxyacetic acid(MCPA), which in the salt form could yield only small amounts of hydrochloric acid, was inconsistent.

Concentrations of 2,4-D required to give an acid reaction in media with varying concentrations of yeast extract were determined the production of about 0.24 mmol of hydrochloric acid seems necessary to counteract the buffering effect of 100 mg of metabolized yeast extract. Both indicator systems show the production of acid, presumably hydrochloric, during degradation of the 2,4-D in the media. Plates of the eosin-methylene blue agar indicate individual 2,4-D-degrading bacterial colonies. A bromocresol purple liquid indicator medium and an eosin-methylene blue agar have been developed for the demonstration and isolation of microorganisms able to degrade the chlorinated herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D).

What is another indicator found in dnase media